Klebsiella Selective Agar

[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]

Klebsiella Selective Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................26.0g
DL –Phenylalanine........................................................................10.0g
L- Ornithine·HCl...........................................................................10.0g
Raffinose.......................................................................................7.0g
Pancreatic digest of casein............................................................2.5g
Yeast extract..................................................................................2.5g
K 2 HPO 4 .........................................................................................2.0g
Phenol Red solution.................................................................10.0mL
Carbenicillin solution...............................................................10.0mL
pH 5.6 ± 0.2 at 25°C
Phenol Red Solution:
Composition per 10.0mL:
Phenol Red....................................................................................0.5g
Preparation of Phenol Red Solution: Add Phenol Red to 50%
ethanol and bring volume to 10.0mL. Mix thoroughly.
Carbenicillin Solution:
Composition per 1.0mL:
Carbenicillin...............................................................................5.0mg
Preparation of Carbenicillin Solution:  Add carbenicillin to dis-
tilled/deionized water and bring volume to 1.0mL. Mix thoroughly. Fil-
ter sterilize.
Preparation of Medium: Add components, except carbenicillin so-
lution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL carbeni-
cillin solution. Mix thoroughly. Adjust pH to 5.6–5.7 with sterile 1N
HCl. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and identification of Klebsiella pneumoniae
from clinical specimens.