Kligler Iron Agar

[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]

Kligler Iron Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone........................................................................................20.0g
Agar ............................................................................................12.0g
Lactose........................................................................................10.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................3.0g
Yeast extract..................................................................................3.0g
Glucose .........................................................................................1.0g
Ferric citrate..................................................................................0.3g
Na 2 S 2 O 3 ........................................................................................0.3g
Phenol Red..................................................................................0.05g
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-

agnostic Systems and Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the differentiation and identification of Enterobacteriaceae
based upon sugar fermentation and hydrogen sulfide production. Sugar
fermentation is indicated by the medium turning yellow. H 2 S produc-
tion results in the medium turning black.