Klebsiella Medium (m-Klebsiella Medium)

[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]

Klebsiella Medium
(m-Klebsiella Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1041.0mL:

Agar............................................................................................15.0g
Adonitol........................................................................................4.0g
2X Salt solution.....................................................................500.0mL
Uric acid solution...................................................................200.0mL
Phenol Red solution.................................................................10.0mL
Sodium taurocholate solution..................................................30.0mL
Carbenicillin solution.................................................................1.0mL
2X Salt Solution:
Composition per liter:
KCl................................................................................................8.0g
K 2 HPO 4 .........................................................................................3.0g
NaCl..............................................................................................2.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
Preparation of 2X Salt Solution:  Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
Uric Acid Solution:
Composition per 200.0mL:
Uric acid........................................................................................0.3g
Preparation of Uric Acid Solution:  Dissolve uric acid in a small
volume of 1N NaOH. Bring volume to 200.0mL with distilled/deion-
ized water. Adjust pH to 7.1 with 1N HCl. Filter sterilize.
Phenol Red Solution:
Composition per 10.0mL:
Phenol Red....................................................................................0.1g
Preparation of Phenol Red Solution:  Add Phenol Red to sterile
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly.
Sodium Taurocholate Solution:
Composition per 30.0mL:
Sodium taurocholate.....................................................................0.4g
Preparation of Sodium Taurocholate Solution: Add sodium
taurocholate to sterile distilled/deionized water and bring volume to
30.0mL. Mix thoroughly.
Carbenicillin Solution:
Composition per 1.0mL:
Carbenicillin ..............................................................................5.0mg
Preparation of Carbenicillin Solution:  Add carbenicillin to dis-
tilled/deionized water and bring volume to 1.0mL. Mix thoroughly. Fil-

ter sterilize.

Preparation of Medium: Add adonitol and agar to 500.0mL of 2X
salt solution. Bring volume to 800.0mL with distilled/deionized water.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 200.0mL
of uric acid solution, 30.0mL of sodium taurocholate solution, 10.0mL
of Phenol Red solution, and 1.0mL of carbenicillin solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the enumeration of Klebsiella species by the membrane filter
method.