Kleb Agar (m-Kleb Agar)

[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]

Kleb Agar
(m-Kleb Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Proteose peptone No. 3...............................................................10.0g
NaCl..............................................................................................5.0g
Adonitol........................................................................................5.0g
Beef extract...................................................................................1.0g
Aniline Blue..................................................................................0.1g
Sodium lauryl sulfate....................................................................0.1g
Phenol Red................................................................................0.025g
Ethanol (95% solution)............................................................20.0mL
Carbenicillin solution...............................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Carbenicillin Solution:
Composition per 10.0mL:
Carbenicillin................................................................................0.05g
Preparation of Carbenicillin Solution: Add carbenicillin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except ethanol and car-
benicillin solution, to distilled/deionized water and bring volume to
970.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add 20.0mL of ethanol and 10.0mL of carbenicillin solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the enumeration of bacteria from waters.