Kirchner Medium Base, Modified

[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]

Kirchner Medium Base, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

L -Asparagine.................................................................................5.0g
KH 2 PO 4 .........................................................................................4.0g
Na 2 HPO 4 .......................................................................................3.0g
Sodium citrate...............................................................................2.5g
MgSO 4 ·7H 2 O................................................................................0.6g
Horse serum, sterile inactivated.............................................100.0mL
Selective supplement solution .................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Penicllin...............................................................................100,000U
Amphotericin B..........................................................................5.0mg
Preparation of Selective Supplement Solution:  Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except horse serum
and selective supplement solution, to distilled/deionized water and
bring volume to 890.0mL. Mix thoroughly. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 50°C. Aseptically add horse serum and se-
lective supplement solution. Mix thoroughly. Pour into Petri dishes or
aseptically distribute into sterile tubes.
Use: For the cultivation of Mycobacterium tuberculosis.