Kirchner Enrichment Medium

[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]

Kirchner Enrichment Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 ·12H 2 O.........................................................................19.0g
Asparagine....................................................................................5.0g
KH 2 PO 4 .........................................................................................2.5g
Sodium citrate...............................................................................2.5g
MgSO 4 ..........................................................................................0.6g
Serum.....................................................................................100.0mL
Glycerol ...................................................................................20.0mL
Penicillin solution....................................................................10.0mL
Phenol Red (0.4% solution).......................................................3.0mL
pH 7.4–7.6 at 25°C
Penicillin Solution:
Composition per 10.0mL:
Penicillin..............................................................................100,000U
Preparation of Penicillin Solution: Add penicillin to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except serum and pen-
icillin solution, to distilled/deionized water and bring volume to
890.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile serum and penicillin solution. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use: For the cultivation and enrichment of Mycobacterium species.