- 定做培养基/定制培养基
- 颗粒培养基
- 标准菌株生化鉴定试剂盒
- 预灌装即用型成品培养基
- 2025年版中国药典
- 促销/特价商品
- 院感/疾控/体外诊断/采样管
- 样品采集与处理(均质)产品
- 按标准检索培养基
- 模拟灌装用培养基
- 干燥粉末培养基
- 培养基添加剂/补充剂
- 生化反应鉴定管
- 染色液等配套产品
- 对照培养基/标准品
- 实验耗材与器具
- 生化试剂/化学试剂
- 菌种鉴定服务
培养基配方
您现在的位置： 网站首页 >> 培养基配方
K-L Virulence Agar (Klebs-Loeffler Virulence Agar) (Elek Agar) (Corynebacterium diphtheriae Virulence Test Medium)
[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]
K-L Virulence Agar
(Klebs-Loeffler Virulence Agar)
(Elek Agar)
(Corynebacterium diphtheriae Virulence Test Medium)
Rabbit serum..........................................................................200.0mL
K 2 TeO 3 solution.....................................................................100.0mL
K-L filter strips..............................................................................100
pH 7.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
K-L Agar Base:
Composition per liter:
Meat peptone ..............................................................................20.0g
Agar............................................................................................15.0g
NaCl..............................................................................................2.5g
Preparation of K-L Agar Base: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C.
K 2 TeO 3 Solution:
Composition per 100.0mL:
K 2 TeO 3 ..........................................................................................0.3g
Preparation of K 2 TeO 3 Solution: Add K 2 TeO 3 to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Caution: Potassium tellurite is toxic.
K-L Filter Strips:
Composition :
Whatman No. 3 filter paper..................................................as needed
Diphtheria toxin solution.........................................................10.0mL
Preparation of K-L Strips: Cut Whatman No. 3 filter paper into
1.5cm × 7cm strips. Autoclave for 15 min at 15 psi pressure–121°C.
Aseptically dip each strip into a sterile solution containing 1000U of
purified diphtheria toxin/mL. Drain off excess liquid.
Preparation of Medium: Filter sterilize rabbit serum. To 1.0L of
cooled, sterile K-L agar base, aseptically add sterile rabbit serum and
sterile K 2 TeO 3 solution. Mix thoroughly. Pour into sterile Petri dishes
in 13.0mL volumes. Before the agar solidifies, aseptically add one K-
L filter strip across the diameter of the plate. Allow the filter strip to
sink to the bottom of the plate or press it down with sterile forceps. Al-
low the agar to solidify. Dry the surface of the plates by incubating at
35°C with lid of plate ajar for 2 hr.
Use: For in vitro toxigenicity testing of Corynebacterium diphtheriae
by the agar diffusion technique. Corynebacterium diphtheriae that pro-
duce toxin form white precipitin lines at approximately 45° angles
from the culture streak line.
(Klebs-Loeffler Virulence Agar)
(Elek Agar)
(Corynebacterium diphtheriae Virulence Test Medium)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per 1300.0mL:
K-L agar base................................................................................1.0LRabbit serum..........................................................................200.0mL
K 2 TeO 3 solution.....................................................................100.0mL
K-L filter strips..............................................................................100
pH 7.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
K-L Agar Base:
Composition per liter:
Meat peptone ..............................................................................20.0g
Agar............................................................................................15.0g
NaCl..............................................................................................2.5g
Preparation of K-L Agar Base: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C.
K 2 TeO 3 Solution:
Composition per 100.0mL:
K 2 TeO 3 ..........................................................................................0.3g
Preparation of K 2 TeO 3 Solution: Add K 2 TeO 3 to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Caution: Potassium tellurite is toxic.
K-L Filter Strips:
Composition :
Whatman No. 3 filter paper..................................................as needed
Diphtheria toxin solution.........................................................10.0mL
Preparation of K-L Strips: Cut Whatman No. 3 filter paper into
1.5cm × 7cm strips. Autoclave for 15 min at 15 psi pressure–121°C.
Aseptically dip each strip into a sterile solution containing 1000U of
purified diphtheria toxin/mL. Drain off excess liquid.
Preparation of Medium: Filter sterilize rabbit serum. To 1.0L of
cooled, sterile K-L agar base, aseptically add sterile rabbit serum and
sterile K 2 TeO 3 solution. Mix thoroughly. Pour into sterile Petri dishes
in 13.0mL volumes. Before the agar solidifies, aseptically add one K-
L filter strip across the diameter of the plate. Allow the filter strip to
sink to the bottom of the plate or press it down with sterile forceps. Al-
low the agar to solidify. Dry the surface of the plates by incubating at
35°C with lid of plate ajar for 2 hr.
Use: For in vitro toxigenicity testing of Corynebacterium diphtheriae
by the agar diffusion technique. Corynebacterium diphtheriae that pro-
duce toxin form white precipitin lines at approximately 45° angles
from the culture streak line.
