King’s OF HiVeg Medium Base with Carbohydrate

[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]

King’s OF HiVeg Medium Base with Carbohydrate

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar..............................................................................................0.3g
Plant hydrolysate ..........................................................................0.2g
Phenol Red.................................................................................3.0mg
Carbohydrate solution............................................................100.0mL
pH 6.9 ± 0.2 at 35°C
Source: This medium, without carbohydrate solution, is available as
a premixed powder from HiMedia.
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate...............................................................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Adonitol, ara-
binose, cellobiose, glucose, dulcitol, fructose, galactose, inositol, lac-
tose, maltose, mannitol, raffinose, rhamnose, salicin, sorbitol, sucrose,
trehalose, xylose, or other carbohydrates may be used. Mix thoroughly.
Filter sterilize.
Preparation of Medium:  Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C. Aseptically add 100.0mL of sterile carbohydrate solution. Mix
thoroughly. Aseptically distribute into tubes.
Use: For studyling oxidation fermentation of carbohydrates by
Campylobacter species.