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King’s OF Basal Medium (Oxidation-Fermentation Medium, King’s) (King’s OF Medium) (BAM M70)
[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]
King’s OF Basal Medium
(Oxidation-Fermentation Medium, King’s)
(King’s OF Medium)
(BAM M70)
Pancreatic digest of casein............................................................2.0g
Carbohydrate solution............................................................100.0mL
Phenol Red (1.5% solution).......................................................2.0mL
pH to 7.3 ± 0.2
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate...............................................................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL
of sterile carbohydrate solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For differentiating bacteria based upon determining the oxidative
and fermentative metabolism of carbohydrates. Bacteria that ferment
the carbohydrate turn the medium yellow.
(Oxidation-Fermentation Medium, King’s)
(King’s OF Medium)
(BAM M70)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar..............................................................................................3.0gPancreatic digest of casein............................................................2.0g
Carbohydrate solution............................................................100.0mL
Phenol Red (1.5% solution).......................................................2.0mL
pH to 7.3 ± 0.2
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate...............................................................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL
of sterile carbohydrate solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For differentiating bacteria based upon determining the oxidative
and fermentative metabolism of carbohydrates. Bacteria that ferment
the carbohydrate turn the medium yellow.
