King’s Medium B

[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]

King’s Medium B

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone No. 3...............................................................20.0g
Agar ............................................................................................15.0g
K 2 HPO 4 .........................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................1.5g
Glycerol ...................................................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Pseudomonas glumae.