Kim-Goepfert Agar (KG Agar)

[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]

Kim-Goepfert Agar
(KG Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

Solution A..............................................................................900.0mL
Egg yolk emulsion, 50%........................................................100.0mL
Polymyxin B solution ................................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Solution A:
Composition per 900.0mL:
Agar ............................................................................................18.0g
Peptone..........................................................................................1.0g
Yeast extract..................................................................................0.5g
Phenol Red................................................................................0.025g
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 900.0mL. Mix thoroughly. Adjust pH to 6.8.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Egg Yolk Emulsion, 50%:
Composition per 100.0mL:
Chicken egg yolks............................................................................11
Whole chicken egg.............................................................................1
NaCl (0.9% solution)...............................................................50.0mL
Preparation of Egg Yolk Emulsion, 50%: Soak eggs with 1:100
dilution of saturated mercuric chloride solution for 1 min. Crack eggs
and separate yolks from whites. Mix egg yolks with 1 chicken egg.
Beat to form emulsion. Measure 50.0mL of egg yolk emulsion and add
to 50.0mL of 0.9% NaCl solution. Mix thoroughly. Filter sterilize.
Warm to 45°–50°C.
Polymyxin B Solution:
Composition per 5.0mL:
Polymyxin B sulfate.............................................................500,000U
Preparation of Polymyxin B Solution: Add polymyxin B to dis-
tilled/deionized water and bring volume to 5.0mL. Mix thoroughly. Fil-
ter sterilize.
Preparation of Medium:  To 900.0mL of cooled, sterile solution A,
aseptically add 100.0mL of sterile egg yolk emulsion, 50%, and 1.0mL
of sterile polymyxin B solution. Mix thoroughly. Pour into sterile Petri
dishes. Allow plates to dry in the dark at 30°C for 24 hr before using.
Use: For the cultivation and differentiation of Bacillus cereus.