Kievskaya Broth with n-Hexadecane

[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]

Kievskaya Broth
with n-Hexadecane

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

KNO 3 ............................................................................................1.0g
KH 2 PO 4 ........................................................................................1.0g
K 2 HPO 4 ........................................................................................1.0g
NaCl..............................................................................................1.0g
MgSO 4 ..........................................................................................0.2g
CaCl 2 ..........................................................................................0.02g
FeCl 3 ..........................................................................................1.0mg
n-Hexadecane ..........................................................................10.0mL

pH 6.8–7.0 at 25°C

Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Gordona terrae, Rhodococcus erythropo-
lis, Rhodococcus luteus, and Rhodococcus maris.