Kanamycin L Broth Medium

[所属分类:培养基配方] [发布时间:2021-4-22] [发布人:李佳颐] [阅读次数:] [返回]

Kanamycin L Broth Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................10.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................5.0g
Glucose.........................................................................................1.0g
Kanamycin solution.................................................................10.0mL
Kanamycin Solution:
Composition per 10.0mL:
Kanamycin...............................................................................50.0mg
Preparation of Kanamycin Solution: Add kanamycin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except kanamycin so-
lution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Bring pH to 7.0. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 10.0mL of sterile kanamycin solution. Mix
thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Escherichia coli.