Kanamycin Luria Agar

[所属分类:培养基配方] [发布时间:2021-4-22] [发布人:李佳颐] [阅读次数:] [返回]

Kanamycin Luria Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Pancreatic digest of casein..........................................................10.0g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................0.5g
Glucose solution......................................................................20.0mL
Kanamycin solution.................................................................10.0mL
Glucose Solution:
Composition per 100.0mL:
Glucose .......................................................................................10.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Kanamycin Solution:
Composition per 10.0mL:
Kanamycin...............................................................................10.0mg
Preparation of Kanamycin Solution: Add kanamycin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except glucose solu-
tion and kanamycin solution, to distilled/deionized water and bring
volume to 970.0mL. Mix thoroughly. Bring pH to 7.0. Autoclave for
15 min at 15 psi pressure–121°C. Aseptically add 20.0mL of sterile
glucose solution and 10.0mL of sterile kanamycin solution. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Escherichia coli.