Kanamycin Esculin Azide HiVeg Broth Base with Kanamycin

[所属分类:培养基配方] [发布时间:2021-4-22] [发布人:李佳颐] [阅读次数:] [返回]

Kanamycin Esculin Azide HiVeg Broth Base
with Kanamycin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Plant hydrolysate ........................................................................20.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................5.0g
Esculin..........................................................................................1.0g
Sodium citrate...............................................................................1.0g
Ferric ammonium citrate...............................................................0.5g
NaN 3 ...........................................................................................0.15g
Kanamycin sulfate solution .....................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Source: This medium, without kanamycin sulfate solution, is avail-
able as a premixed powder from HiMedia.
Kanamycin Sulfate Solution:
Composition per 10.0mL:
Kanamycin sulfate......................................................................0.02g
Preparation of Kanamycin Sulfate Solution: Add kanamycin
sulfate to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of enterococci from foods.