IUT Medium Base with Glycerol and Egg Emulsion

[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]

IUT Medium Base with Glycerol and Egg Emulsion

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1600.0mL:

L -Asparagine.................................................................................3.6g
KH 2 PO 4 .......................................................................................2.46g
Magnesium citrate ........................................................................0.6g
Malachite Green............................................................................0.4g
MgSO 4 ·7H 2 O..............................................................................0.24g
Egg emulsion ................................................................................1.0L
Glycerol ...................................................................................12.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Egg Emulsion:
Composition per 1.0L:
Eggs ........................................................................................Variable
Preparation of Egg Emulsion:  Soak whole eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack eggs. Beat
to form emulsion. Avoiding the formation of air bubbles. Sterilize by
inspissation at 85°C for 1 hr.
Preparation of Medium: Add glycerol to distilled/deionized water
and bring volume to 600.0mL. Mix thoroughly. Add remaining com-
ponents other than egg emulsion. Gently heat and bring to boiling. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Add sterile
whole egg emulsion. Aseptically distribute into culture vessels.
Use: For the cultivation of Mycobacterium tuberculosis.