J Agar

[所属分类:培养基配方] [发布时间:2021-4-22] [发布人:李佳颐] [阅读次数:] [返回]

J Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Yeast extract................................................................................15.0g
Pancreatic digest of casein............................................................5.0g
K 2 HPO 4 .........................................................................................3.0g
Glucose solution ......................................................................10.0mL
pH 7.3–7.5 at 25°C
Glucose Solution:
Composition per 10.0mL:
Glucose .........................................................................................2.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile glu-
cose solution. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the cultivation of Bacillus species and Sporolactobacillus
species.