编号：
批号：

培养基配方

您现在的位置：网站首页 >> 培养基配方

ITC HiVeg Broth Base with Ticarcillin and Potassium Chlorate (TTC HiVeg Broth Base)

[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]

ITC HiVeg Broth Base
with Ticarcillin and Potassium Chlorate
(TTC HiVeg Broth Base)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

MnCl 2 ·6H 2 O...............................................................................60.0g
Plant hydrolysate ........................................................................10.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................1.0g
Malachite Green..........................................................................0.01g
Irgansan (Trichlosan).................................................................1.0mg
Ticarcillin solution...................................................................10.0mL
Potassium chlorate solution
pH 6.9 ± 0.2 at 25°C
Source: This medium, without ticarcillin and potassium chlorate, is
available as a premixed powder from HiMedia.
Ticarcillin Solution:
Composition per 10.0mL:
Ticarcillin...................................................................................1.0mg
Preparation of Ticarcillin Solution: Add ticarcillin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Poassium Chlorate Solution:
Composition per 10.0mL:
KClO 3 ...........................................................................................1.0g
Preparation of Poassium Chlorate Solution: Add KClO 3 to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Caution: Potassium chlorate is a highly oxidizable agent and can
cause explosions. Take proper precautions when handling.
Preparation of Medium: Add components, except ticarcillin and
potassium chlorate solutions, to distilled/deionized water and bring
volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 10.0mL ticarcillin solution and 10.0mL
potassium chlorate solution. Mix thoroughly. Distribute into tubes or
flasks.
Use: For the selective isolation and cultivation of Yersinia species. For
the selective enrichment and enumeration of Yersinia enterocolitica.

在线客服

ITC HiVeg Broth Base with Ticarcillin and Potassium Chlorate (TTC HiVeg Broth Base)