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培养基配方
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ISP Medium 6 (International Streptomyces Project Medium 6) (Peptone Yeast Extract Iron Agar)
[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]
ISP Medium 6
(International Streptomyces Project Medium 6)
(Peptone Yeast Extract Iron Agar)
Peptone .......................................................................................15.0g
Proteose peptone...........................................................................5.0g
K 2 HPO 4 .........................................................................................1.0g
Yeast extract..................................................................................1.0g
Ferric ammonium citrate...............................................................0.5g
Na 2 S 2 O 3 ......................................................................................0.08g
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Streptomyces species.
(International Streptomyces Project Medium 6)
(Peptone Yeast Extract Iron Agar)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar............................................................................................15.0gPeptone .......................................................................................15.0g
Proteose peptone...........................................................................5.0g
K 2 HPO 4 .........................................................................................1.0g
Yeast extract..................................................................................1.0g
Ferric ammonium citrate...............................................................0.5g
Na 2 S 2 O 3 ......................................................................................0.08g
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Streptomyces species.
