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培养基配方
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ISP Medium 5 (International Streptomyces Project Medium 5) (Glycerol Asparagine Agar)
[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]
ISP Medium 5
(International Streptomyces Project Medium 5)
(Glycerol Asparagine Agar)
Glycerol ......................................................................................10.0g
L- Asparagine.................................................................................1.0g
K 2 HPO 4 .........................................................................................1.0g
Trace salts solution ....................................................................1.0mL
pH 7.4 ± 0.2 at 25°C
Trace Salts Solution:
Composition per 100.0mL:
FeSO 4 ·7H 2 O..................................................................................0.1g
MnCl 2 ·4H 2 O .................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
Preparation of Trace Salts Solution: Add components to dis-
tilled/deionized water and bring the volume to 100.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except trace salts solu-
tion, to distilled/deionized water and bring volume to 999.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 1.0mL of
sterile trace salts solution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the cultivation and maintenance of Pseudonocardia species
and Streptomyces peucetius.
(International Streptomyces Project Medium 5)
(Glycerol Asparagine Agar)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar ............................................................................................20.0gGlycerol ......................................................................................10.0g
L- Asparagine.................................................................................1.0g
K 2 HPO 4 .........................................................................................1.0g
Trace salts solution ....................................................................1.0mL
pH 7.4 ± 0.2 at 25°C
Trace Salts Solution:
Composition per 100.0mL:
FeSO 4 ·7H 2 O..................................................................................0.1g
MnCl 2 ·4H 2 O .................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
Preparation of Trace Salts Solution: Add components to dis-
tilled/deionized water and bring the volume to 100.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except trace salts solu-
tion, to distilled/deionized water and bring volume to 999.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 1.0mL of
sterile trace salts solution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the cultivation and maintenance of Pseudonocardia species
and Streptomyces peucetius.
