ISP Medium 9 (International Streptomyces Project Medium 9)

[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]

ISP Medium 9
(International Streptomyces Project Medium 9)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

K 2 HPO 4 ·3H 2 O ............................................................................5.65g
(NH 4 ) 2 SO 4 ..................................................................................2.64g
KH 2 PO 4 .......................................................................................2.38g
MgSO 4 ·7H 2 O................................................................................1.0g
Carbohydrate solution............................................................100.0mL
Pridham and Gottlieb trace salts................................................1.0mL
pH 6.8–7.0 at 25°C
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate...............................................................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL.Use glucose,
arabinose, sucrose, xylose, inositol, mannitol, fructose, rhamnose,
raffinose, or cellulose. Mix thoroughly. Filter sterilize.
Pridham And Gottlieb Trace Salts:
Composition per 100.0mL:
MnCl 2 ·7H 2 O................................................................................0.79g
CuSO 4 ·5H 2 O...............................................................................0.64g
ZnSO 4 ·7H 2 O...............................................................................0.15g
FeSO 4 ·7H 2 O................................................................................0.11g
Preparation of Pridham and Gottlieb Trace Salts: Add com-
ponents to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat and bring to boiling with frequent agita-
tion. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add sterile carbohydrate solution. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and differentiation of Streptomyces purpureus
and other Streptomyces species based on carbohydrate utilization.