Intracellular Growth Phase Medium (IGP Medium)

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]

Intracellular Growth Phase Medium
(IGP Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 100.0mL:

Gentamicin sulfate.................................................................500.0mg
Lysozyme.................................................................................30.0mg
Eagle MEM..............................................................................72.0mL
Dulbecco’s phosphate-buffered saline.....................................20.0mL
Fetal bovine serum.....................................................................8.0mL
pH 7.2–7.4 at 25°C
Eagle MEM:
Composition per liter:
NaCl..............................................................................................8.0g
Glucose.........................................................................................1.0g
KCl................................................................................................0.4g
CaCl 2 ·2H 2 O ................................................................................0.14g
MgSO 4 ·7H 2 O................................................................................0.1g
KH 2 PO 4 .......................................................................................0.06g
Na 2 HPO 4 .....................................................................................0.05g
L- Isoleucine...............................................................................0.026g
L- Leucine ..................................................................................0.026g
L- Lysine.....................................................................................0.026g
L- Threonine...............................................................................0.024g
L- Valine...................................................................................0.0235g
L- Tyrosine .................................................................................0.018g
L- Arginine...............................................................................0.0174g
L- Phenylalanine.......................................................................0.0165g
L- Cystine...................................................................................0.012g
L- Histidine..................................................................................8.0mg
L- Methionine..............................................................................7.5mg
Phenol Red.................................................................................5.0mg
L- Tryptophan..............................................................................4.0mg
Inositol.......................................................................................1.8mg
Biotin.........................................................................................1.0mg
Folic acid ...................................................................................1.0mg
Calcium pantothenate ................................................................1.0mg
Choline chloride.........................................................................1.0mg
Nicotinamide..............................................................................1.0mg
Pyridoxal·HCl............................................................................1.0mg
Thiamine·HCl ............................................................................1.0mg
Riboflavin..................................................................................0.1mg
Preparation of Eagle MEM: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly.
Dulbecco’s Phosphate-Buffered Saline:
Composition per liter:
NaCl..............................................................................................8.0g
Na 2 HPO 4 ·7H 2 O...........................................................................2.16g
KCl................................................................................................0.2g
KH 2 PO 4 .........................................................................................0.2g
CaCl 2 .............................................................................................0.1g
MnCl 2 ·6H 2 O .................................................................................0.1g
Preparation of Dulbecco’s Phosphate-Buffered Saline: Add
components to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly.
Preparation of Medium:  Combine components. Mix thoroughly.
Filter sterilize. Aseptically distribute into sterile tubes or flasks.
Use: For the screening of Escherichia coli for pathogenicity using the
HeLa cell test for invasiveness.