Ion Agar for Ureaplasma

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]

Ion Agar for Ureaplasma

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 101.45mL:

HEPES (N-[2-hydroxyethyl]piperazine-N′-[2-ethane-
sulfonic acid]) buffer............................................................1.19g
Ionagar No. 2..............................................................................0.75g
Pancreatic digest of casein............................................................0.7g
NaCl..............................................................................................0.5g
Beef extract...................................................................................0.3g
Yeast extract..................................................................................0.3g
Beef heart, solids from infusion....................................................0.2g
Yeast extract..................................................................................0.1g
Horse serum, normal sterile.....................................................10.0mL
Ampicillin solution....................................................................1.0mL
Urea solution............................................................................0.25mL
Nystatin solution........................................................................0.1mL
Tripeptide solution.....................................................................0.1mL
pH 7.2 ± 0.2 at 25°C
Ampicillin Solution:
Composition per 10.0mL:
Ampicillin.....................................................................................1.0g
Preparation of Ampicillin Solution:  Add ampicillin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Urea Solution:
Composition per 100.0mL:
Urea.............................................................................................10.0g
Preparation of Urea Solution:  Add urea to distilled/deionized wa-
ter and bring volume to 100.0mL. Filter sterilize. Store at –20°C.
Nystatin Solution:
Composition per 1.0mL:
Nystatin..................................................................................50,000U
Preparation of Nystatin Solution: Add nystatin to distilled/de-
ionized water and bring volume to 1.0mL. Filter sterilize.
Tripeptide Solution:
Composition per 10.0mL:
Glycyl- L- histidyl- L- lysine acetate ..............................................0.2mg
Preparation of Tripeptide Solution: Add glycyl- L- histidyl- L-
lysine acetate to distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. Filter sterilize. Store at –20°C.
Preparation of Medium: Add components—except horse serum,
ampicillin solution, urea solution, nystatin solution, and tripeptide so-
lution—to distilled/deionized water and bring volume to 90.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of
sterile horse serum, 1.0mL of sterile ampicillin solution, 0.25mL of
sterile urea solution, 0.1mL of sterile nystatin solution, and 0.1mL of
sterile tripeptide solution. Mix thoroughly. Pour into sterile Petri dish-
es.
Use: For the cultivation of Ureaplasma species from clinical specimens.