Inositol Urea Caffeic Acid Medium

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]

Inositol Urea Caffeic Acid Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar solution .........................................................................900.0mL
Base solution..........................................................................100.0mL
Agar Solution:
Composition per 900.0mL:
Agar............................................................................................15.0g
Preparation of Agar Solution: Add agar to distilled/deionized
water and bring volume to 900.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Base Solution:
Composition per 100.0mL:
Inositol........................................................................................10.0g
Urea...............................................................................................5.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
Caffeic acid...................................................................................0.2g
NaCl..............................................................................................0.1g
CaCl 2 ·2H 2 O ..................................................................................0.1g
Gentamicin sulfate......................................................................0.04g
H 3 BO 3 ........................................................................................0.5mg
ZnSO 4 ·7H 2 O..............................................................................0.4mg
MnSO 4 ·4H 2 O.............................................................................0.4mg
Thiamine·HCl............................................................................0.4mg
Pyroxidine·HCl..........................................................................0.4mg
Niacin.........................................................................................0.4mg
Calcium pantothenate ................................................................0.4mg
p-Aminobenzoic acid.................................................................0.2mg
Riboflavin..................................................................................0.2mg
FeCl 3 ..........................................................................................0.2mg
Na 2 MoO 4 ·4H 2 O.........................................................................0.2mg
KI...............................................................................................0.1mg
CuSO 4 ·5H 2 O............................................................................0.04mg
Folic acid ....................................................................................2.0μg
Biotin..........................................................................................2.0μg
Ferric citrate solution (1% solution)..........................................1.0mL
Preparation of Base Solution:  Add components, except urea, to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Gently heat just until components are dissolved. Cool to 75°–80°C.
Add urea. Mix thoroughly. Do not heat after addition of urea. Do not

autoclave. Filter sterilize.

Preparation of Medium: Aseptically combine the cooled, sterile

agar solution with the sterile base solution. Mix thoroughly. Pour into
sterile Petri dishes.
Use: For the selective isolation and differentiation of Cryptococcus
species based on inositol and urea utilization and pigment production
from caffeic acid. On this medium, only Cryptococcus species utilize
inositol as sole carbon source and urea as sole nitrogen source. Cryp-

tococcus neoformans appears as dark brown colonies. Other Crypto-

coccus species are unpigmented.