Inorganic Salt Medium (Modified Raggios Medium)

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]

Inorganic Salt Medium
(Modified Raggios Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 CO 3 .........................................................................................3.0g
KI................................................................................................0.75g
MgSO 4 ·7H 2 O................................................................................0.7g
CaCl 2 ·2H 2 O...............................................................................0.446g
KH 2 PO 4 .........................................................................................0.2g
Na 2 SO 4 ..........................................................................................0.2g
KCl............................................................................................0.165g
MnSO 4 ·2H 2 O...........................................................................6.64mg
ZnSO 4 ·7H 2 O............................................................................2.67mg
FeCl 3 ·4H 2 O................................................................................2.5mg
H 3 BO 3 ........................................................................................1.5mg
Na 2 MoO 4 ·2H 2 O .......................................................................0.25mg
CuSO 4 ·5H 2 O............................................................................0.07mg

Source: This medium is available from HiMedia.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of soil microorganisms such as Rhizobium

spp.