Inositol Assay Medium

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]

Inositol Assay Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.....................................................................................100.0g
Potassium citrate.........................................................................10.0g
Citric acid......................................................................................2.0g
KH 2 PO 4 .........................................................................................1.1g
KCl..............................................................................................0.85g
L- Asparagine.................................................................................0.8g
L- Glutamic acid.............................................................................0.6g
L- Isoleucine...................................................................................0.5g
L- Leucine ......................................................................................0.5g
L- Lysine.........................................................................................0.5g
L- Valine.........................................................................................0.5g
L- Arginine...................................................................................0.48g
DL- Alanine ....................................................................................0.4g
DL- Threonine.................................................................................0.4g
CaCl 2 ...........................................................................................0.25g
MgSO 4 ·7H 2 O..............................................................................0.25g
DL- Aspartic acid............................................................................0.2g
DL- Phenylalanine ..........................................................................0.2g
Glycine..........................................................................................0.2g
L- Methionine.................................................................................0.2g
L- Tyrosine .....................................................................................0.2g
L- Proline........................................................................................0.2g
L- Histidine.................................................................................0.124g
DL- Serine.......................................................................................0.1g
L- Cystine.......................................................................................0.1g
DL- Tryptophan.............................................................................0.08g
FeCl 3 ...........................................................................................0.05g
MnSO 4 ·7H 2 O..............................................................................0.05g
Calcium pantothenate ................................................................5.0mg
Pyridoxine·HCl..........................................................................1.0mg
Thiamine·HCl ............................................................................0.5mg
Biotin.......................................................................................0.01mg
pH 5.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 5 min at 15 psi pressure–121°C.
Use: For the microbiological assaying of inositol using Saccharomy-
ces uvarum as the test organism.