Inorganic Salts Maltose Medium

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]

Inorganic Salts Maltose Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract..................................................................................4.0g
Peptone..........................................................................................2.0g
Inorganic salts solution..........................................................980.0mL
Maltose solution.......................................................................20.0mL
pH 7.5 ± 0.2 at 25°C
Inorganic Salts Solution:
Composition per liter:
MgSO 4 ·7H 2 O............................................................................49.37g
NaCl............................................................................................43.8g
CaCl 2 ·2H 2 O.................................................................................1.29g
Preparation of Inorganic Salts Solution: Add NaCl first and
then other components to distilled/deionized water and bring volume
to 1.0L. Mix thoroughly.
Maltose Solution:
Composition per 100.0mL:
Maltose........................................................................................25.0g
Preparation of Maltose Solution: Add maltose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except maltose solu-
tion, to inorganic salts solution and bring volume to 980.0mL. Mix
thoroughly. Adjust pH to 7.5 with KOH. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 25°C. Aseptically add 20.0mL of sterile malt-
ose solution. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use:  For the cultivation and maintenance of Spirochaeta halophila.