Inorganic Salts-Maltose Medium (DSMZ Medium 754)

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]

Inorganic Salts-Maltose Medium
(DSMZ Medium 754)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract..................................................................................4.0g
Peptone .........................................................................................2.0g
Inorganic salt solution............................................................980.0mL
Maltose solution.......................................................................20.0mL
Maltose Solution:
Composition per liter:
Maltose .........................................................................................5.0g
Preparation of Maltose Solution: Add maltose to 20.0mL dis-
tilled/deionized water. Mix thoroughly. Filter sterilize.
Inorganic Salt Solution:
Composition per liter:
MgSO 4 ·7H 2 O............................................................................49.37g
NaCl............................................................................................43.8g
CaCl 2 ·2H 2 O ................................................................................1.29g
Preparation of Inorganic Salt Solution: Add components in the
order CaCl 2 ·2H 2 O, NaCl, MgSO 4 ·7H 2 O to 900.0mL distilled/deionized
water. After addition of each, mix thorougly to prevent precipitation.
Add distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components except maltose solution
to 980.0mL inorganic salt solution. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 20.0mL
sterile maltose solution. Mix thoroughly. Aseptically distribute to ster-
ile tubes or flasks.
Use:  For the cultivation of Spirochaeta halophila.