Imidazole Utilization Medium

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]

Imidazole Utilization Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Imidazole ......................................................................................5.0g
KH 2 PO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCl 2 ..........................................................................................3.0mg
FeSO 4 ·7H 2 O...............................................................................3.0mg
Molybdenum solution................................................................1.0mL
Trace elements solution.............................................................1.0mL
pH 6.0 ± 0.2 at 25°C
Molybdenum Solution:
Composition per 18.0mL:
Na 2 MoO 4 ·2H 2 O.........................................................................0.5mg
Preparation of Molybdenum Solution: Add components to dis-

tilled/deionized water and bring volume to 18.0mL. Mix thoroughly.

Filter sterilize.

Trace Elements Solution:
Composition per 18.0mL:
H 3 BO 3 ......................................................................................11.0mg
MnCl 2 ·4H 2 O...............................................................................7.0mg
Al 2 (SO 4 ) 3 ·18 H 2 O....................................................................1.94mg
Co(NO 3 ) 2 ·6H 2 O .........................................................................1.0mg
CuSO 4 ·5H 2 O..............................................................................1.0mg
NiSO 4 ·6H 2 O...............................................................................1.0mg
ZnSO 4 ·H 2 O..............................................................................0.62mg
KBr.............................................................................................0.5mg
KI...............................................................................................0.5mg
LiCl............................................................................................0.5mg
SnCl 2 ·2H 2 O................................................................................0.5mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 18.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except molybdenum
solution and trace elements solution, to distilled/deionized water and
bring volume to 998.0mL. Mix thoroughly. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Aseptically add 1.0mL of molybdenum solution and 1.0mL of trace el-
ements solution. Mix thoroughly. Adjust pH to 6.0 with phosphoric ac-
id. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Pseudomonas species.