Imhoff’s Medium, Modified

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]

Imhoff’s Medium, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................30.0g
NaHCO 3 ........................................................................................3.0g
KH 2 PO 4 .........................................................................................1.0g
NH 4 Cl ...........................................................................................1.0g
Sodium acetate..............................................................................1.0g
Na 2 SO 4 ..........................................................................................0.7g
MgCl 2 ·6H 2 O..................................................................................0.5g
Sodium ascorbate..........................................................................0.5g
CaCl 2 ·2H 2 O...................................................................................0.1g
Yeast extract..................................................................................0.1g
Sodium sulfide solution...........................................................10.0mL
SLA trace elements solution......................................................1.0mL
VA vitamin solution...................................................................1.0mL
pH 6.9–7.0 at 25°C
Sodium Sulfide Solution:
Composition per 100.0mL:

Na 2 S·9H 2 O....................................................................................2.0g

Preparation of Sodium Sulfide Solution: Add Na 2 S·9H 2 O to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C under N 2 . Maintain
under 100% N 2 .
SLA Trace Elements Solution:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.8g
H 3 BO 3 ...........................................................................................0.5g
CoCl 2 ·6H 2 O................................................................................0.25g
ZnCl 2 .............................................................................................0.1g
MnCl 2 ·4H 2 O ...............................................................................0.07g
Na 2 MoO 4 ·2H 2 O..........................................................................0.03g
CuCl 2 ·2H 2 O................................................................................0.01g
Na 2 SeO 3 ·5H 2 O............................................................................0.01g
NiCl 2 ·6H 2 O.................................................................................0.01g
Preparation of SLA Trace Elements Solution: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust pH to 2–3. Filter sterilize.
VA Vitamin Solution:
Composition per 500.0mL:
Nicotinamide...............................................................................0.17g
Thiamine·HCl.............................................................................0.15g
p-Aminobenzoic acid....................................................................0.1g
Biotin..........................................................................................0.05g
Calcium pantothenate .................................................................0.05g
Pyridoxine·2HCl.........................................................................0.05g
Cyanocobalamin.........................................................................0.02g
Preparation of VA Vitamin Solution: Add components to dis-
tilled/deionized water and bring volume to 500.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components—except sodium sulfide
solution, SLA trace elements solution, and VA vitamin solution—to
distilled/deionized water and bring volume to 988.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Asep-
tically add 1.0mL of sterile SLA trace elements solution and 1.0mL of
sterile VA vitamin solution. Aseptically add 10.0mL of sterile sodium
sulfide solution. Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.
Use: For the cultivation and maintenance of Rhodobacter adriaticus
and Rhodobacter sulfidophilus.