Ilyobacter Broth

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]

Ilyobacter Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................20.0g
MgCl 2 ·6H 2 O .................................................................................3.0g
KCl................................................................................................0.5g
NH 4 Cl .........................................................................................0.25g
KH 2 PO 4 .........................................................................................0.2g
CaCl 2 ·2H 2 O ................................................................................0.15g
Resazurin ...................................................................................1.0mg
Sodium sulfide solution...........................................................10.0mL
Sodium  L- tartrate solution........................................................10.0mL
NaHCO 3 solution.....................................................................10.0mL
Trace elements solution SL-7....................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Sodium Sulfide Solution:
Composition per 100.0mL:
Na 2 S·9H 2 O....................................................................................3.6g
Preparation of Sodium Sulfide Solution: Add Na 2 S·9H 2 O to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C under 100% N 2 .
Maintain under 100% N 2 .
Sodium  L- Tartrate Solution:
Composition per 10.0mL:
Sodium  L- tartrate...........................................................................2.0g
Preparation of Sodium  L- Tartrate Solution: Add sodium  L- tar-
trate to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize. Maintain under 80% N 2 + 20% CO 2 .
NaHCO 3 Solution:
Composition per 10.0mL:
NaHCO 3 ........................................................................................2.5g
Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize. Maintain under 80% N 2 + 20% CO 2 .
Trace Elements Solution SL-7:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O................................................................................0.19g
MnCl 2 ·4H 2 O .................................................................................0.1g
ZnCl 2 ...........................................................................................0.07g
H 3 BO 3 .......................................................................................0.062g
Na 2 MoO 4 ·2H 2 O ........................................................................0.036g
NiCl 2 ·6H 2 O...............................................................................0.024g
CuCl 2 ·2H 2 O ..............................................................................0.017g
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution SL-7: Add the
FeCl 2 ·4H 2 O to the HCl. Add distilled/deionized water and bring vol-
ume to 1.0L. Add remaining components. Mix thoroughly. Filter ster-
ilize. Maintain under 80% N 2 + 20% CO 2 .
Preparation of Medium: Add components—except sodium sulfide
solution, sodium  L- tartrate solution, NaHCO 3 solution, and trace ele-
ments solution SL-7—to distilled/deionized water and bring volume to
969.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Maintain un-
der 80% N 2 + 20% CO 2 . Aseptically add 10.0mL of sodium  L- tartrate
solution, 10.0mL of NaHCO 3 solution, and 1.0mL of trace elements
solution SL-7 under 80% N 2 + 20% CO 2 . Mix thoroughly. Aseptically
distribute into sterile tubes or flasks under 80% N 2 + 20% CO 2 . Adjust
pH to 7.2. At time of inoculation add sodium sulfide solution to a final
concentration of 0.1%.
Use:  For the cultivation and maintenance of Ilyobacter tartaricus.