Ilyobacter Agar

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]

Ilyobacter Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................20.0g
Agar ............................................................................................15.0g
MgCl 2 ·6H 2 O..................................................................................3.0g
KCl................................................................................................0.5g
NH 4 Cl .........................................................................................0.25g
KH 2 PO 4 .........................................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.15g
Resazurin ...................................................................................1.0mg
Sodium sulfide solution...........................................................10.0mL
Sodium  L- tartrate solution........................................................10.0mL
NaHCO 3 solution.....................................................................10.0mL
Trace elements solution SL-7 ....................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Sodium Sulfide Solution:
Composition per 100.0mL:
Na 2 S·9H 2 O....................................................................................3.6g
Preparation of Sodium Sulfide Solution: Add Na 2 S·9H 2 O to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C under N 2 . Maintain
under 100% N 2 .
Sodium  L -Tartrate Solution:
Composition per 10.0mL:
Sodium  L -tartrate ..........................................................................2.0g
Preparation of Sodium  L- Tartrate Solution: Add sodium  L- tar-
trate to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize. Maintain under 80% N 2 + 20% CO 2 .
NaHCO 3 Solution:
Composition per 10.0mL:
NaHCO 3 ........................................................................................2.5g
Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize. Maintain under 80% N 2 + 20% CO 2 .
Trace Elements Solution SL-7:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O ................................................................................0.19g
MnCl 2 ·4H 2 O..................................................................................0.1g
ZnCl 2 ...........................................................................................0.07g
H 3 BO 3 .......................................................................................0.062g
Na 2 MoO 4 ·2H 2 O ........................................................................0.036g
NiCl 2 ·6H 2 O...............................................................................0.024g
CuCl 2 ·2H 2 O ..............................................................................0.017g
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution SL-7: Add the
FeCl 2 ·4H 2 O to the HCl. Add distilled/deionized water and bring vol-
ume to 1.0L. Add remaining components. Mix thoroughly. Filter ster-
ilize. Maintain under 80% N 2 + 20% CO 2 .
Preparation of Medium: Add components—except agar, sodium
sulfide solution, sodium  L- tartrate solution, NaHCO 3 solution, and
trace elements solution SL-7—to distilled/deionized water and bring
volume to 469.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Maintain under 80% N 2 + 20% CO 2 . Aseptically add 10.0mL of sodi-
um  L- tartrate solution, 10.0mL of NaHCO 3  solution, and 1.0mL of
trace elements solution SL-7 under 80% N 2 + 20% CO 2 . Mix thorough-
ly. In a separate flask, add agar to distilled/deionized water and bring
volume to 500.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Combine sterile agar and sterile basal medium. Adjust pH to 7.2. Asep-
tically add 10.0mL of sodium sulfide solution. Pour into sterile Petri
dishes or distribute into sterile tubes. Maintain under 80% N 2 + 20%
CO 2 .
Use:  For the cultivation and maintenance of Ilyobacter tartaricus.