Ilyobacter Medium

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]

Ilyobacter Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Crotonic acid.................................................................................1.7g
NaCl..............................................................................................1.0g
Yeast extract..................................................................................1.0g
Na 2 HPO 4 ·12H 2 O...........................................................................0.7g
KCl................................................................................................0.5g
MgCl 2 ·6H 2 O..................................................................................0.4g
NH 4 Cl ...........................................................................................0.3g
Na 2 SO 4 ..........................................................................................0.1g
Sodium sulfide solution...........................................................10.0mL
CaCl 2 ·2H 2 O (1.0% )...................................................................1.0mL
FeCl 3 (0.5% ).............................................................................1.0mL
Modified SL-7 trace elements solution......................................1.0mL
Resazurin (0.1% ) ......................................................................1.0mL
Selenite-tungstate solution.........................................................1.0mL
pH 6.8–7.2 at 25°C
Sodium Sulfide Solution:
Composition per 100.0mL:
Na 2 S·9H 2 O....................................................................................3.6g
Preparation of Sodium Sulfide Solution: Add Na 2 S·9H 2 O to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C under N 2 . Maintain
under 100% N 2 .
Modified SL-7 Trace Elements Solution:
Composition per liter:
CoCl 2 ·6H 2 O ..................................................................................0.2g
MnCl 2 ·4H 2 O..................................................................................0.1g
ZnCl 2 ...........................................................................................0.07g
H 3 BO 3 .........................................................................................0.06g
Na 2 MoO 4 ·2H 2 O ..........................................................................0.04g
CuCl 2 ·2H 2 O ................................................................................0.02g
NiCl 2 ·6H 2 O.................................................................................0.02g
HCl (1N) ....................................................................................3.0mL
Preparation of Modified SL-7 Trace Elements Solution: Add
components to distilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Filter sterilize. Maintain under 80% N 2 + 20% CO 2 

Selenite-Tungstate Solution:

Composition per liter:
NaOH............................................................................................0.5g
Na 2 WO 4 ·2H 2 O...........................................................................4.0mg
Na 2 SeO 3 ·5HO............................................................................3.0mg
Preparation of Selenite-Tungstate Solution: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize. Maintain under 80% N 2 + 20% CO 2 .
Preparation of Medium: Add components—except sodium sulfide
solution, modified SL-7 trace elements solution, and selenite-tungstate
solution—to distilled/deionized water and bring volume to 969.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Adjust pH to 5.5. Cool to 45°–50°C under
100% N 2 . Maintain under 100% N 2 . Aseptically add 1.0mL of sterile
modified SL-7 trace elements solution and 1.0mL of sterile selenite-
tungstate solution under 100% N 2 . Mix thoroughly. Aseptically distrib-
ute into sterile tubes or flasks under 100% N 2 . At time of inoculation,
add sodium sulfide solution to a final concentration of 1.0%. Maintain
under 100% N 2 .
Use:  For the cultivation and maintenance of Ilyobacter delafieldii.