Ignisphaera Medium (DSMZ Medium 1043)

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]

Ignisphaera Medium
(DSMZ Medium 1043)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Trypticase peptone........................................................................2.0g
Starch, soluble...............................................................................2.0g
(NH 4 ) 2 SO 4 ....................................................................................1.3g
MgSO 4 · 7H 2 O..............................................................................0.28g
KH 2 PO 4 ......................................................................................0.28g
Yeast extract..................................................................................0.1g
L -Cysteine.....................................................................................0.3g
CaCl 2 ·2H 2 O .............................................................................74.0mg
Resazurin...................................................................................0.5mg
FeCl 2 · 6H 2 O................................................................................0.5mg
Trace elements solution...........................................................10.0mL
FeCl 2  solution ..........................................................................10.0mL
Na 2 S·9H 2 O solution.................................................................10.0mL
pH 6.5 ± 0.2 at 25°C
FeCl 2 Solution :
Composition per 10.0mL:
FeCl 2 · 6H 2 O................................................................................0.5mg
Preparation of FeCl 2 Solution:  Add FeCl 2 · 6H 2 O to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Sparge
with 100% N 2 . Filter sterilize.
Na 2 S·9H 2 O Solution :
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................0.3g
Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave under 100% N 2 for 15 min at 15 psi pressure–121°C. Cool
to room temperature.
Trace Elements Solution:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O................................................................................0.5g
CoSO 4 ·7H 2 O...............................................................................0.18g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CaCl 2 ·2H 2 O ..................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·4H 2 O..........................................................................0.01g
CuSO 4 ·5H 2 O...............................................................................0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution:  Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except iron chloride
and sulfide solutions, to distilled/deionized water and bring volume to
980.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 1
min. Cool to room temperature while sparging with 100% N 2 . Add
FeCl 3 solution. Adjust pH to 6.3. Dispense under an atmosphere of
100% N 2 into suitable culture vessels (e.g., aliquots of 20mL medium
into 50mL serum bottles). Autoclave for 15 min at 15 psi pressure–
121°C. Cool to room temperature. Adjust pH to 6.5. Prior to inocula-
tion aseptically and anoxically add sulfide solution.
Use: For the cultivation of Ignisphaera spp.