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Ignicoccus Medium (DSMZ Medium 897)

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]
Ignicoccus Medium
(DSMZ Medium 897)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..........................................................................................13.65g
Sulfur, powdered...........................................................................5.0g
MgSO 4 ·7H 2 O................................................................................3.5g
MgCl 2 ·6H 2 O................................................................................2.75g
Meat extract ..................................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
CaCl 2 ·2H 2 O.................................................................................0.38g
KCl..............................................................................................0.33g
(NH 4 ) 2 SO 4 ...................................................................................0.25g
NaBr............................................................................................0.05g
H 3 BO 3 ......................................................................................15.0mg
SrCl 3 ·6H 2 O...............................................................................7.50mg
KI............................................................................................ 0.05mg
Resazurin ...................................................................................0.5mg
Na 2 S·9H 2 O solution.................................................................10.0mL
pH 5.5 ± 0.2 at 25°C
Na 2 S·9H 2 O Solution:
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................0.2g
Preparation of Na 2 S·9H 2 O Solution:  Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Sparge with N 2 .
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Store an-
aerobically.
Preparation of Medium: Add components, except Na 2 S·9H 2 O so-
lution, to distilled/deionized water and bring volume to 990.0mL.
Sparge medium with N 2 gas for 30–60 min. Mix thoroughly. Add
10.0mL Na 2 S·9H 2 O solution. Mix thoroughly. Adjust pH to 5.5 with
H 2 SO 4 . Distribute into tubes or bottles under 80% H 2 and 20% CO 2 gas

mixture. Heat the vessels containing medium in boiling water for 1 hr

before inoculation. After inoculation pressurize the vessels with 80%

H 2 and 20% CO 2 gas mixture to 2 bar overpressure.

Use:  For the cultivation of Ignicoccus islandicus and Ignicoccus pacifi-

cus.


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