IFO Agar

[所属分类:培养基配方] [发布时间:2021-4-19] [发布人:李佳颐] [阅读次数:] [返回]

IFO Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
(NH 4 ) 2 HPO 4 ..................................................................................3.0g
NaCl..............................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
FeSO 4 ·7H 2 O.............................................................................10.0mg
MnSO 4 ·6H 2 O.............................................................................5.0mg
Riboflavin................................................................................0.02mg
Calcium pantothenate ..............................................................0.02mg
Pyridoxine·HCl........................................................................0.02mg
Nicotinic acid...........................................................................0.02mg
p-Aminobenzoic acid...............................................................0.01mg
Thiamine·HCl ..........................................................................0.01mg
Biotin..........................................................................................1.0μg
Methanol..................................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except agar and meth-
anol, to distilled/deionized water and bring volume to 490.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. In a separate flask, add agar to distilled/deionized water and
bring volume to 500.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically combine the two sterile solutions. Aseptically add
10.0mL of filter-sterilized methanol. Mix thoroughly. Adjust pH to 7.0.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Hyphomicrobium methy-
lovorum.