IE Medium

[所属分类:培养基配方] [发布时间:2021-4-19] [发布人:李佳颐] [阅读次数:] [返回]

IE Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1011.0mL:

Agar ............................................................................................15.0g
Peptone..........................................................................................5.0g
Yeast extract..................................................................................1.0g
Basal salts solution........................................................................1.0L
Lactose solution..........................................................................10.0g
Trace elements solution .............................................................1.0mL
Basal Salts Solution:
Composition per liter:
MgSO 4 ..........................................................................................0.5g
Phosphate solution...................................................................20.0mL
(NH 4 )SO 4  (36% solution) ..........................................................5.0mL
Phosphate Solution:
Composition per liter:
K 2 HPO 4 .......................................................................................95.0g
NaH 2 PO 4 ·2H 2 O...........................................................................78.0g
Preparation of Phosphate Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Basal Salts Medium: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad-
just pH to 6.8-7.0.
Lactose Solution:
Composition per 10.0mL:
Lactose..........................................................................................2.5g
Preparation of Lactose Solution:  Add lactose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize. Warm to 50°–55°C.
Trace Elements Solution:
Composition per liter:
Disodium EDTA ...........................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.2g
H 3 BO 3 .........................................................................................0.03g
CoCl 2 ·6H 2 O................................................................................0.02g
ZnSO 4 ·7H 2 O...............................................................................0.01g
MnCl 2 ·4H 2 O ..............................................................................3.0mg
Na 2 MoO 4 ·2H 2 O.........................................................................3.0mg
NiCl 2 ·6H 2 O................................................................................2.0mg
CaCl 2 ·2H 2 O ...............................................................................1.0mg
Preparation of Trace Elements Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Combine components, except lactose so-
lution and trace elements solution. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°–55°C. Aseptically add 10.0mL of sterile lactose solution and
1.0mL of sterile trace elements solution. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Bacillus species.