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IFO Broth

[所属分类:培养基配方] [发布时间:2021-4-19] [发布人:李佳颐] [阅读次数:] [返回]
IFO Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

(NH 4 ) 2 HPO 4 ..................................................................................3.0g
NaCl..............................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
FeSO 4 ·7H 2 O.............................................................................10.0mg
MnSO 4 ·6H 2 O.............................................................................5.0mg
Riboflavin.................................................................................20.0μg
Calcium pantothenate ...............................................................20.0μg
Pyridoxine·HCl.........................................................................20.0μg
Nicotinic acid............................................................................20.0μg
p-Aminobenzoic acid................................................................10.0μg
Thiamine·HCl ...........................................................................10.0μg
Biotin ..........................................................................................1.0μg
Methanol..................................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except methanol, to
distilled/deionized water and bring volume to 990.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add
10.0mL of filter-sterilized methanol. Mix thoroughly. Adjust pH to 7.0.
Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Hyphomicrobium methy-
lovorum.
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