Halomonas desiderata Medium (LMG Medium 271)

[所属分类:培养基配方] [发布时间:2021-3-29] [发布人: ] [阅读次数:] [返回]

Halomonas desiderata Medium

(LMG Medium 271)

培养基配方

Composition per liter:
Glucose.........................................................................................5.0g
KNO 3 ............................................................................................2.0g
KH 2 PO 4 .........................................................................................1.0g
MgCl 2 ·6H 2 O .................................................................................0.2g
Na 2 CO 3 / NaHCO 3 solution.....................................................100.0mL
pH 9.5–10.0
Na 2 CO 3 /NaHCO 3 Solution:
Composition per 100.0mL:
Na 2 CO 3 .........................................................................................5.4g
NaHCO 3 ........................................................................................4.2g
Preparation of Na 2 CO 3 /NaHCO 3 Solution: Add NaHCO 3 and Na 2 CO 3  to 100.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Add components, except Na 2 CO 3 /NaHCO 3  solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 100.0mL sterile Na 2 CO 3 /NaHCO 3 solution. Pour into sterile Petri dishes or aseptically distribute into sterile tubes.
Use: For cultivation and maintenance of Halomonas desiderata.