Halomonas magadiensis Medium (DSMZ Medium 971)

[所属分类:培养基配方] [发布时间:2021-3-29] [发布人: ] [阅读次数:] [返回]

Halomonas magadiensis Medium

(DSMZ Medium 971)

培养基配方

Composition per liter:
Agar ............................................................................................20.0g
Glucose .......................................................................................10.0g
Peptone..........................................................................................5.0g
Yeast extract..................................................................................5.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
NaCl solution.........................................................................200.0mL
Na 2 CO 3 solution.....................................................................100.0mL
pH 10.0 ± 0.2 at 25°C
NaCl Solution:
Composition per 200.0mL:
NaCl............................................................................................40.0g
Preparation of NaCl Solution:  Add NaCl to distilled/deionized water and bring volume to 200.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 60°C.
Na 2 CO 3 Solution:
Composition per 100.0mL:
Na 2 CO 3 .......................................................................................10.0g
Preparation of Na 2 CO 3 Solution:  Add Na 2 CO 3  to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 60°C. 

Preparation of Medium: Add components, except NaCl solution and Na 2 CO 3 solution, to distilled/deionized water and bring volume to 700.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 60°C. Aseptically add 200.0mL NaCl solution and 100.0mL Na 2 CO 3 solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Halomonas magadiensis.