Halomonas desiderata Medium (DSMZ Medium 762)

[所属分类:培养基配方] [发布时间:2021-3-29] [发布人: ] [阅读次数:] [返回]

Halomonas desiderata Medium

(DSMZ Medium 762)

培养基配方

Composition per liter:
Glucose.........................................................................................5.0g
KNO 3 ............................................................................................2.0g
KH 2 PO 4 .........................................................................................1.0g
MgCl 2 ·6H 2 O .................................................................................0.2g
Carbonate solution.................................................................100.0mL
pH 9.5-10.0 at 25°C
Carbonate Solution
Composition per 100.0mL:
Na 2 CO 3 .........................................................................................5.4g
NaHCO 3 ........................................................................................4.2g
Preparation of Carbonate Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except carbonate solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add 100.0mL sterile carbonate solution. Mix thoroughly. Aseptically distribute into sterile tubes or bottles.
Use:  For the cultivation of Halomonas desiderata.