Halomicrobium katesii Medium (DSMZ Medium 1164)

[所属分类:培养基配方] [发布时间:2021-3-29] [发布人: ] [阅读次数:] [返回]

Halomicrobium katesii Medium (DSMZ Medium 1164)

培养基配方

Composition per liter:

NaCl..........................................................................................195.0g
MgSO 4 · 7H 2 O............................................................................49.83g
Proteose peptone...........................................................................5.0g
Yeast extract .................................................................................5.0g
KCl................................................................................................5.0g
Glucose.........................................................................................1.0g
CaCl 2 ·2H 2 O ..............................................................................0.916g
NaBr............................................................................................0.35g
NaHCO 3 ....................................................................................0.166g
FeCl 3 ·6H 2 O, 0.5% soltuion...................................................... 7 drops
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Halomicrobium katesii.