Acetate HiVeg Agar

[所属分类:培养基配方] [发布时间:2019-9-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetate HiVeg Agar
Composition per liter:
Sodium acetate·3H 2 O................................................................27.22g
Agar............................................................................................20.0g
Glucose.......................................................................................10.0g
Yeast extract..................................................................................5.0g
Plant peptone ................................................................................5.0g
Plant extract No.1.........................................................................5.0g
Tween™ 80................................................................................0.5mL
pH 5.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically adjust pH to 5.4. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the isolation and cultivation of Leuconostoc species and
Pediococcus species.