Acetate Differential Agar (Sodium Acetate Agar) (Simmons’ Citrate Agar, Modified)

[所属分类:培养基配方] [发布时间:2019-9-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetate Differential Agar
(Sodium Acetate Agar)
(Simmons’ Citrate Agar, Modified)
Composition per liter:
Agar............................................................................................20.0g
NaCl..............................................................................................5.0g
Sodium acetate..............................................................................2.0g
(NH 4 )H 2 PO 4 ..................................................................................1.0g
K 2 HPO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
Bromthymol Blue.......................................................................0.08g
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to cold distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Gently heat and
bring to boiling. Distribute into tubes to produce a 1 cm butt and 30 cm
slant. Autoclave for 15 min at 15 psi pressure–121°C. Cool tubes in a
slanted position.
Use: For the differentiation of Shigella species from Escherichia coli
and also for the differentiation of nonfermenting Gram-negative bacte-
ria. Bacteria that can utilize acetate as the sole carbon source turn the
medium blue.