Acetic Acid Agar

[所属分类:培养基配方] [发布时间:2019-9-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetic Acid Agar
Composition per liter:
Agar............................................................................................25.0g
Sodium acetate, anhydrous...........................................................4.5g
Yeast extract..................................................................................1.0g
Wort solution..........................................................................500.0mL
Sucrose solution.....................................................................500.0mL
Wort Solution:
Composition per 500.0mL:
Malt extract.................................................................................55.0g
Preparation of Wort Solution : Add malt extract to distilled/deion-
ized water and bring volume to 500.0mL. Mix thoroughly.
Sucrose Solution:
Composition per 500.0mL:
Sucrose........................................................................................50.0g
Preparation of Sucrose Solution : Add sucrose to distilled/deion-
ized water and bring volume to 500.0mL. Mix thoroughly.
Preparation of Medium:  Combine components. Mix thoroughly.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation and maintenance of Tetragenococcus halo-
philus.