Acetate Agar (BAM M3)

[所属分类:培养基配方] [发布时间:2019-9-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetate Agar
(BAM M3)
Composition per liter:
Agar ............................................................................................20.0g
NaCl..............................................................................................5.0g
Sodium acetate..............................................................................2.0g
KH 2 PO 4 .........................................................................................1.0g
(NH 4 ) 2 PO 4 ....................................................................................1.0g
MgSO 4 ..........................................................................................0.2g
Bromthymol Blue .......................................................................0.08g
pH 6.7 ± 0.2 at 25°C
Preparation of Medium: Add components, except MgSO 4 , to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen-
tly heat and bring to boiling. Adjust pH to 6.7. Add 0.2g MgSO 4 . Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically distribute into tubes or flasks. For slants distrib-
ute aliquots into tubes prior to autoclaving. After autoclaving allow
tubes to cool in inclined position to obtain a 5cm slant.
Use : For the cultivation of Leuconostoc species.