GYM + Seawater (DSMZ Medium 871)

[所属分类:培养基配方] [发布时间:2021-3-22] [发布人: ] [阅读次数:] [返回]

GYM + Seawater

(DSMZ Medium 871)

培养基配方

Composition per liter:
Sea salts ......................................................................................32.0g
Agar............................................................................................15.0g
Malt extract.................................................................................10.0g
Glucose.........................................................................................4.0g
Yeast extract..................................................................................4.0g
CaCO 3 ...........................................................................................2.0g
pH 7.0–7.4 at 25°C
Preparation of Medium: Add sea salts to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Add remaining components. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Nocardiopsis aegyptia (Nocardiopsis sp.) and Nocardiopsis halotolerans (Nocardiopsis sp.).