GYM Starch Agar (DSMZ Medium 214)

[所属分类:培养基配方] [发布时间:2021-3-22] [发布人: ] [阅读次数:] [返回]

GYM Starch Agar

(DSMZ Medium 214)

培养基配方

Composition per liter:
Starch..........................................................................................20.0g
Agar............................................................................................12.0g
Malt extract.................................................................................10.0g

Glucose.........................................................................................4.0g

Yeast extract..................................................................................4.0g
CaCO 3 ...........................................................................................2.0g
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components, except agar, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Add agar. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Amycolatopsis orientalis subsp. orientalis, Nocardia spp., Mycobacterium spp., Pseudonocardia spp., Saccharothrix spp., Kineosporia aurantiaca, Kitasatospora setae, Oerskovia turbata (Cellulomonas turbata), Cellulosimicrobium cellulans, and Thermoactinomyces dichotomicus.