GYM+S Agar

[所属分类:培养基配方] [发布时间:2021-3-22] [发布人: ] [阅读次数:] [返回]

GYM+S Agar

培养基配方

Composition per liter:
Starch..........................................................................................20.0g
Agar............................................................................................12.0g
Malt extract.................................................................................10.0g
CaCO 3 ...........................................................................................4.0g
Glucose.........................................................................................4.0g
Yeast extract..................................................................................4.0g
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components, except agar, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Using pH indicator paper, adjust pH to 7.2 with KOH. Add agar. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Actinomadura species, Actinoplanes species, Amycolata autotrophica, Amycolatopsis orientalis, Amycolatopsis sulphurea, Cellulomonas cellulans, Cellulomonas turbata, Gordona rubropertinctus, Kineosporia aurantiaca, Mycobacterium species, Nocardia species, Nocardioides albus, Nocardiopsis albus, Oerskovia species, Promicromonospora enterophila, Pseudonocardia compacta, Saccharomonospora viridis, Saccharopolyspora hirsuta, Saccharothrix coeruleofusca, Saccharothrix longispora, Sporichthya polymorpha, Streptomyces species, Streptosporangium corrugatum, and Thermoactinomyces dichotomicus.