Acetamide Medium (BAM M2)

[所属分类:培养基配方] [发布时间:2019-9-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetamide Medium
(BAM M2)
Composition per liter:
Stock basal solution...............................................................400.0mL
Stock acetamide solution .......................................................100.0mL
pH 6.9 ± 0.2 at 25°C
Stock Basal Solution:
Composition per 400.0mL:
Agar..............................................................................................0.5g
KH 2 PO 4  solution, 0.5M...........................................................14.0mL
K 2 HPO 4  solution, 0.5M.............................................................6.0mL
PR-CV solution..........................................................................1.0mL
Preparation of Stock Basal Solution:  Add components, except
PR-CV solution, to distilled/deionized water and bring volume to
400.0mL. Mix thoroughly. Gently heat and bring to boiling with agita-
tion to dissolve agar. Add 1.0mL PR-CV solution.
PR-CV Solution:
Composition per 200.0mL:
Phenol Red....................................................................................2.0g
Crystal Violet................................................................................0.2g
Preparation of PR-CV Solution:  Add components to distilled/de-
ionized water and bring volume to 200.0mL. Mix thoroughly. Add 5N
NaOH while stirring until components are dissolved.
Stock Acetamide Solution:
Composition per 100.0mL:
Acetamide.....................................................................................1.0g
Preparation of Stock Acetamide Solution:  Add acetamide to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Store over methylene chloride in a screw-capped container. Can be
stored indefinitely at room temperature.
Preparation of Medium: Combine 400.0mL stock basal solution
and 100.0mL stock acetamide solution. Mix thoroughly. Distribute into
tubes or flasks. Steam for 10 min at 100°C. Cool.
Use : For the differentiation of nonfermentative Gram-negative bact-
eria, especially Pseudomonas aeruginosa, e.g., in milk.